Detection and quantification of COMP neoepitope in equine saliva: A biomarker for detection of early stages of Osteoarthritis
| dc.contributor.author | Lord, Moa | |
| dc.contributor.department | Chalmers tekniska högskola / Institutionen för fysik | sv |
| dc.contributor.examiner | Karlsteen, Magnus | |
| dc.contributor.supervisor | Nyström, Susanne | |
| dc.contributor.supervisor | Skiöldebrand, Eva | |
| dc.date.accessioned | 2021-06-04T11:54:05Z | |
| dc.date.available | 2021-06-04T11:54:05Z | |
| dc.date.issued | 2021 | sv |
| dc.date.submitted | 2020 | |
| dc.description.abstract | Osteoarthritis (OA) is a chronic in ammatory joint disease, with painful destructive processes locally in the joint. It is not possible to diagnose the early destructive biochemical events within the joint that leads to pain and subsequent lameness. Many horses with local joint pain due to early OA with a low-grade in ammation are used today in the equestrian industry. This disease develops over time and is usually not diagnosed until the end stages, where irreversible damage and clinical symptoms can be noticed. A biomarker for early matrix degradation of articular cartilage is needed as a diagnostic tool for early diagnosis of OA. A speci c neoepitope of cartilage oligomeric matrix protein (COMP) have previously been identi ed and shown to increase in synovial uid and serum from horses with acute lameness. The aim of this thesis was to detect and quantify the speci c COMP neoepitope in saliva sampled from healthy horses and horses with OA. This aim included evaluation of sample collection and preparation, sample collection throughout the day and validation of the capillary western blot Wes™by inhibition ELISA. Salivette®, a speci c collection device developed for salivary collection, was used to collect the saliva. Saliva sampled on the horse tongue, no food intake within one hour, obtained lower background noise, showed higher chemiluminescence signal and more de ned peaks compared to saliva sampled close to food intake, when analysed with Wes™. In Wes™, the COMP neoepitope could be detected at the apparent molecular weight of 58 kDa. The concentration of the COMP neoepitope was determined with inhibition ELISA developed for quanti cation of COMP neoepitope in synovial uid and serum from horses. The highest concentration of COMP neoepitope was quanti ed in a horse recently diagnosed with OA, compared to saliva from healthy horses. This indicates that saliva from horses can be used for detection and quanti cation of COMP neoepitope. However, this thesis included a limited amount of horses and they were not clinically examined by a veterinarian prior to saliva sampling. A larger group of well-de ned healthy horses and horses with OA is required to draw statistical conclusions. Quanti cation of COMP neoepitope in saliva is a step toward the future goal of developing a point of care diagnostics, which can be an accessible tool for diagnosis of OA, monitoring the horse during rehabilitation and also be used in everyday training of athletic horses. | sv |
| dc.identifier.coursecode | TIFX61 | sv |
| dc.identifier.uri | https://hdl.handle.net/20.500.12380/302399 | |
| dc.language.iso | eng | sv |
| dc.setspec.uppsok | PhysicsChemistryMaths | |
| dc.subject | Osteoarthritis | sv |
| dc.subject | Biomarker | sv |
| dc.subject | COMP neoepitope | sv |
| dc.subject | Saliva | sv |
| dc.subject | Wes™ | sv |
| dc.subject | Inhibition ELISA | sv |
| dc.title | Detection and quantification of COMP neoepitope in equine saliva: A biomarker for detection of early stages of Osteoarthritis | sv |
| dc.type.degree | Examensarbete för masterexamen | sv |
| dc.type.uppsok | H | |
| local.programme | Biotechnology (MPBIO), MSc |